Analysis Note

ControlWhole cell lysate from mouse embryonic stem cells.

Application

Research CategoryStem Cell Research

Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

Immunogen

Synthetic peptide from mouse Nanog.

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

Quality

Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 µg of Embryonic Mouse Brain lysates

Specificity

Antibody recognizes Nanog.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Target description

39 kDa (MW 34 kDa)